Purification Propensity for Proteins from Bacillus halodurans

The demand for proteins with special purposes increases significantly, for example, special proteins are in good need in development of sensitive, specific and reliable differential diagnostic assays. To meet such huge demand, proteins of interest can be expressed in either prokaryotic or eukaryotic cells, like Escherichia coli, to produce recombinant proteins. However, this is not an easy task and is often costly. Purification ofrecombinant proteins from plant biomass currently accounts for almost 80% ofproduction cost. A series of difficulties may be encountered in purification. For instance, purified proteins from a host may accumulate in low titers and may be mixed with infection or form protein aggregates. Therefore a purification scheme usually includes many steps, such as affinity chromatography, precipitation, protecting of recombinant proteins from degradation with stabilizer, centrifugation and so on.

In order to develop an efficient and cost-effective purification scheme, many efforts have been made and much has been achieved infinding the factors that affect protein purification. At codon optimization level, N-terminal rare codons increase expression with some reservation.