Nuclear Magnetic Resonance (NMR) spectroscopy is a nondestructive,
quantitative, reproducible, untargeted and unbiased method that requires no or
minimal sample preparation, and is one of the leading analytical tools for
metabolomics research. The easy quantification and the no need of priorknowledge about compounds present in a sample associated with NMR areadvantageous over other techniques. 1H NMR is especially attractive because
protons are present in virtually all metabolites and its NMR sensitivity is
high, enabling the simultaneous identification and monitoring of a wide range
of low molecular weight metabolites.
However, the resolution of the 1H NMR spectra from intact
tissues is often poor due to the unwanted line broadenings arising from
isotropic magnetic susceptibility variations near boundaries of inter- andintracellular structures, residual homo-nuclear proton dipolar coupling and
residual chemical shift anisotropy interaction.
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