At a time when the demand for the demand renewable is growing at the rate of 30 billion gallons of biofuels by the end of 2020, it is time to explore new technologies that are capable of generating cellulosic ethanol. This can be done by breaking down cellulose into cellulase enzyme. However, this cannot be the mostable and degrades at higher temperatures in bioreactors. A study was conducted to create eco-friendly bioethanol from cellulosic waste recombinant that can be used at higher temperature in bioreactors. The project involved molecular cloning of genes for cellulose-degrading enzymes based on bacterial source, expressing the recombinant proteins in E. coli and optimized the enzymatic activity. The study could generate in vitro bacterial expression systems to produce recombinant His-tag purified protein which showed cellulase like activity.
Cheap, clean, green energy production is a goal of Department of energy and EPA. Biofuels are made by converting renewable materials- -for example, corn kernels, wood chips left over from pulp and paper production, prairie grasses, and even garbage--into fuels and chemicals. Most biofuels used today are made from the fermentation of starch from corn kernels. That process, although simple, is costly because of the high price of the corn kernels themselves.
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