Thursday, 13 April 2017

Gene Profiling of Plant Cell Wall Biosynthesis for Genetic Enhancing Biomass Enzymatic Saccharification in Cereal Crops

Rice, wheat, corn, and sweet sorghum are four major cereal crops around the world. Over the past years, great efforts have been made to improve grain yields by using genetic breeding and biotechnology. For instance, the annual grain yield in 2014 year reached 6.76, 4.96 and 5.87 tons per hectare for rice, wheat, and corn in China (National data: http://data.stats.gov.cn/). Sweet sorghum has been considered as an ideal bioenergy crop for its high fermentable sugar content in stalk and degradable lignocellulose in bagasse with high tolerance to environmental stress. Those four cereal crops can provide large amount of lignocellulose residues, accounting for major biomass resources harvested in the farming fields all over the world. However, biomass residues are currently far from good applications, and alternatively some residues are burned in the field, leading to biomass waste and air pollution.

Enzymatic Saccharification in Cereal CropsAs fossil fuels are constantly used as major energy source, bioenergy has been considered as a renewable energy derived from biomass resources for heat, electricity, fuel and chemical products. Due to food security, however, bioenergy crops could not be allowed to grow in large arable lands, and lignocellulose-based residues of food crops are alternative source of biomass.

Wednesday, 12 April 2017

A Comparison of Glycomics in Prokaryotes and Eukaryotes

Normally glycan cannot be found independently in nature, they will be branched either with proteins known as glycol proteins or branched with lipids called as glycolipids. Glycobiology is an interesting and very complex study when compared to other studies because such complexity cannot be seen in other studies. Molecules for Protein, RNA, DNA, lipids and sugar linkages found in saccharides provide the important structural basis for molecules. These 68 building blocks form an entire life of cell, nucleosides (RNA) or nucleotides (DNA) have four blocks each. Lipids are constituted of 8 categories, which are divided on the basis of Ketoacyl and isoprene. 20 different amino acids and 32 sugar linkages found in saccharides. These all increase the complexity more.

 Prokaryotes and EukaryotesAdding to the complexity many proteins are seen to be involved with glycans, these not only act as carriers of carbohydrates. In some cases these proteins also involves in reacting with carbohydrates.To understand glycans better we should understand the roles of glycans, Glycoproteins is one form of glycans these are found on cell surface playing a vital role of recognition.

Tuesday, 11 April 2017

Vitamin D and Immune System

Vitamin D interaction with immune system is one of the most well-established non-classical effects of Vitamin D. Vitamin D can modulate the innate and adaptive immune responses. The ability of Vitamin D to influence normal human immunity will be highly dependent on the vitamin D status of individuals; therefore, deficiency or insufficiency of Vitamin D is associated with increased autoimmunity and infection. The 25-hydroxyvitamin D3 (25OHD3) is the main circulating metabolite of Vitamin D and is the most reliable measurement of an individual’s Vitamin D status. The Vitamin D supplements in deficient individuals will have beneficial immune-modulatory effects on the autoimmune status.

Vitamin D and Immune System

Vitamin D3 itself is biologically inactive. Thus, after being synthesized in the skin, vitamin D3 binds to the vitamin D-binding protein (DBP) in the blood to be transported into the liver where the first hydroxylation at position 25 occurs producing the major circulating metabolite 25-hydroxy vitamin D3 (25(OH)D3)

Tuesday, 4 April 2017

A True Symbiosis for the Mitochondria Evolution

Endosymbiotic theory (or Symbiogenesis) is an evolutionary theory that was initially proposed more than 100 years ago to explain the origins of eukaryotic cells from the prokaryotic ones. This theory postulated that several key organelles of eukaryotes could have been originated as a symbiosis between separate organisms. Later in the 1920s, this idea was extended to explain the origins of mitochondria and, some 30 years ago, it was definitively accepted. According to this theory, a pro-bacteria was hosted by an eukaryotic cell and evolved within it, providing the host with all the ATP that is needed (therefore acting as a cellular “power house”). Some other studies were confirming this hypothesis by assessing that substantial gene additions were impossible without the energetic boost provided by the colonizing mitochondrion in the eukaryotic lineage.

 Symbiosis for the Mitochondria EvolutionMore recently this idea was questioned by a work in which the authors analyzed the cellular energetics and genomics data from a wide variety of species obtaining an indication that, relatively to the ATP requirements of a cell throughout its life, the costs of a gene at the DNA, RNA, and protein levels decline with cell volume in both bacteria and eukaryotes. These costs are usually sufficiently large to be perceived by natural selection in bacterial populations, but not in eukaryotes experiencing high levels of random genetic drift.

Monday, 3 April 2017

Variety of Neuronal Pathways to Achieve the Same Hypoxic Preconditioning Effect



The relevance of hypoxic preconditioning is due to its ability to increase the body's resistance to hypoxic/ischemic stress. Hypoxic factor is the main inischemic preconditioning effect. It is likely that hypoxic component forms the pathogenesis of many diseases, and a study of the preconditioning mechanisms is a high priority.

Hypoxic Preconditioning Effect
In the problem of hypoxic adaptation, the brain is central, not only as the most sensitive organ to hypoxia, but also as the coordinator of functions of all body organs and systems. In the nervous tissue, the functional specificity and individualsensitivity to hypoxia of separate neuronal populations and the corresponding brain structures is of fundamental importance. The structures of forebrain are most unstable to ischaemic/hypoxic injuries. The most interesting are the cortex and hippocampus, as these are the higher brain structures responsible for cognitive functions and complex behaviour.



Friday, 31 March 2017

A Paediatric Perspective on Stem Cells: Expression, Function and Clinical Relevance

In the last ten years, placenta, fetal membranes (i.e. amnion and chorion), and amniotic fluid have been extensively investigated as a potential non-controversial source of stem cells. They are usually discarded after delivery and are accessible during pregnancy through amniocentesis and chorionic villus sampling Several populations of cells with multi-lineage differentiation potential and immunemodulatory properties have been isolated from the human placenta and fetal membranes; they have been classified by an international workshop as human amniotic epithelial cells (hAECs) human amniotic mesenchymal stromal cells (hAMSCs) human chorionic mesenchymal stromal cells (hCMSCs) by Igura et al. and Anker et al., and human chorionic trophoblastic cells (hCTCs). In the amniotic fluid (AF), two main populations of stem cells have been isolated so far:

Stem Cells
The AF is the clear, watery liquid that surrounds the growing fetus within the amniotic cavity. It allows the fetus to freely grow and move inside the uterus, protects it from outside injuries by cushioning sudden blows or movements by maintaining consistent pressure and temperature, and acts as a vehicle for the exchange of body chemicals with the mother.

Thursday, 30 March 2017

Supported UV Polymerized Ionic Liquid Membranes with Block Copolymer

Room temperature ionic liqulids (RTILs) are useful materials for generating supported ionic liquid membranes (SILM) due to their selectivity for acid gases e.g. CO2 and H2S, low vapor pressure as well as ‘green’ nature. RTILs are reported to have higher mass transport coefficients than traditional membranes generated from a variety of polymeric matrices, thus, leading to higher permeabilities and diffusivities. A number of studies have reported on SILM for separation of CO2/CH4, CO2/N2, olefin/ paraffin, etc. However, this prominent attribute suffers from IL clusters’ fragile nature within the polymeric membranes, thus, leading to their leaching from surface and reduction in performance. An alternative is to polymerize the RTILs either as homopolymers or copolymers. Poly (ionic liquids) (PRTIL) have been demonstrated to possess higher CO2 adsorption capacities than the respective monomers. 

UV Polymerized Ionic Liquid Membranes with Block Copolymer
Separation ability was not reduced at the partial CO2 operating pressures of 207 kPa. Another work investigated the effect of polymerized and ‘free’ RTIL percentage on a composite gel membrane for CO2 separation . Polymerization percentage had different effects on CO2 permeability depending on the RTIL content of the composite membrane. Highest permeability was obtained with RTIL content of 75% (free). Other works have investigated polymerized RTIL/liquid RTIL composite membranes for CO2 separation. A maximum selectivity of 24.9 was reported for CO2 from a mixture with CH4. Such membranes had been extremely selective for CO2 in CO2/CH4 mixtures in previous works as well.