Transcriptomic profiling analyses are frequently used for the study of
cells in response to environmental stress factors that often impede cell
optimal growth. Given that transcriptional profiles of optimally growing cells
differ significantly from those of sub-optimally growing cells, stress-induceddifferentially-transcribed genes are thus inevitably mixed with slow growthgenes.
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| Stress Response |
It is therefore necessary to separate the stress-specific response genes
from slow growth genes. Methodologies used to deconvolute stress-specific
response from non-specific response such as slow growth are discussed in this
editorial. Transcription regulation is one of the major ways to control gene
activity in cells. Hence, transcription levels of genes are thought to be
linked largely to the levels of gene activity. Serial Analysis of Gene
Expression (SAGE) was the technology that for the first time allows the study
of a transcriptome or a majority of transcripts in eukaryotic cells. Instead of
sequencing the entire Expressed Sequence Tag (EST) derived from cDNA libraries,
the SAGE method takes only a ~15 bps tag from end of each cDNA fragment and
ligates to chain multiple short cDNA tags for PCR amplification and sequencing
analysis. Read more>>>>>>>
